Integrase of Mason-Pfizer monkey virus

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F05%3A00016049" target="_blank" >RIV/60461373:22330/05:00016049 - isvavai.cz</a>

Alternative codes found

RIV/61388963:_____/05:00021077

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Integrase of Mason-Pfizer monkey virus

Original language description

The gene encoding an integrase of Mason#Pfizer monkey virus (M-PMV)is located at the 3-end of the pol open reading frame. The M-PMV integrasehas not been previously isolated and characterized. We have nowcloned, expressed, isolated, and characterized M-PMV integrase and comparedits activities and primary structure with those of HIV-1 and otherretroviral integrases. M-PMV integrase prefers untranslated 3-regionderivedlong-terminal repeat sequences in both the 3-processing and thestrand transfer activityassays. While the 3-processing reaction catalyzedby M-PMV integrase was significantly increased in the presence ofMn2+ and Co2+ and was readily detectable in the presence of Mg2+ andNi2+ cations, the strand transfer activity was strictly dependent only onMn2+. M-PMV integrase displays more relaxed substrate specificity thanHIV-1 integrase, catalyzing the cleavage and the strand transfer ofM-PMV and HIV-1 long-terminal repeat-derived substrates with similarefficiency. The structure-based

Czech name

Integrasa Mason-Pfizerova opičího viru

Czech description

Gen kodující integrázu Mason-Pfizerova opičího viru se nachází na 3' konci čtecího rámce pol. M-PMV integráza doposud nebyla izolována a charakterizována. My jsme naklonovali, exprimovali, izolovali a charakterizovali M-PMV integrázu.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

FEBS Journal

ISSN

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e-ISSN

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Volume of the periodical

272

Issue of the periodical within the volume

1

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

14

Pages from-to

203-216

UT code for WoS article

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EID of the result in the Scopus database

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