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Analysis of elements species fractions in buckwheat and amaranth flours by SEC/ICP-MS and MALDI-MS

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F07%3A00019275" target="_blank" >RIV/60461373:22330/07:00019275 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Analysis of elements species fractions in buckwheat and amaranth flours by SEC/ICP-MS and MALDI-MS

  • Original language description

    Fractionation of Co, Cu, Fe, Mn, Mo, Ni, P and Zn species in extracts of buckwheat semolina and amaranth flour in 0.02 moll-1 Tris-HCl (pH=7.5) buffer was performed by SEC/ICP-MS hyphenation. Element specific chromatograms of the buckwheat extract are similar to those of legumes and cereals. Except for Fe and Mn the remaining metals are concentrated in a low-molecular fraction (Mr<2 kDa). Fe is bound in a high-molecular fraction (Mr>150 kDa). Chromatograms of Co, Fe, Mo and Ni species in amaranth extracts correspond with this pattern too. However, Cu is also bound in a medium-molecular fraction (Mr~40 kDa) and in a high-molecular one (Mr>150 kDa). The main part of Mn in both extracts is present in labile complex and ionic form. Fractions rich of tracemetals were isolated using preparative-scale SEC and the organic ligands of metals were consequently preconcentrated on Chelex 100 in Cu2+ form and eluted by ammonia solution. Low content of sulphur containing amino acids (Cys, Met) and h

  • Czech name

    Analýza frakcí prvkových specií v pohankové a amarantové mouce metodami SEC/ICP-MS a MALDI-MS

  • Czech description

    Frakcionace specií Co, Cu, Fe, Mn, Mo, Ni, P a Zn v extraktech pohankové krupice a amarantové mouky v 0,02 mol/l Tris-HCL (pH=7,5) byla provedena metodou SEC/ICP-MS.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    GM - Food industry

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2007

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    European Food Research and Technology

  • ISSN

    1438-2377

  • e-ISSN

  • Volume of the periodical

    225

  • Issue of the periodical within the volume

    5-6

  • Country of publishing house

    DE - GERMANY

  • Number of pages

    10

  • Pages from-to

    895-904

  • UT code for WoS article

  • EID of the result in the Scopus database