Fractionation of phosphorus and trace elements species in soybean flour and common bean seeds by SEC/ICP-MS

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F02%3A00007175" target="_blank" >RIV/60461373:22340/02:00007175 - isvavai.cz</a>

Alternative codes found

RIV/60461373:22330/02:00007175

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Fractionation of phosphorus and trace elements species in soybean flour and common bean seeds by SEC/ICP-MS

Original language description

Soluble species of phosphorus, sulfur, selenium and eight metals (Mn, Fe, Co, Ni, Cu, Zn, Mo and Cd) in soybean flour and common bean seeds were investigated by size exclusion chromatography (SEC) and ICP-MS. Samples were extracted by 0.02 mol l-1 Tris-HCl buffer solution (pH=7.5). Fractionation of sample extracts by preparative scale SEC was accomplished using Fractogel EMD BioSEC column (600 16 mm) and 0.02 mol l-1 Tris-HCl buffer solution (pH=7.5) as a mobile phase (flow rate 2 ml min-1). 2 ml of sample were injected. Contents of elements in chromatographic fractions were determined by AAS, ICP-AES and ICP-MS. The elution profiles of P, Fe, Co, Ni, Cu, Zn and Mo in both samples were similar. Main species of Co, Ni, Cu, Zn and Mo were found in low molecular weight region (2 to 5 kDa), whereas Fe is predominantly bound to high molecular weight compounds (180 kDa). Dominant phosphorus fraction was detected in medium molecular weight region (10 to 30 kDa) and the other one in low molecu

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

<a href="/en/project/GA525%2F00%2F0581" target="_blank" >GA525/00/0581: Speciation analysis of trace elements in foods</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2002

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Chromatography B

ISSN

1570-0232

e-ISSN

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Volume of the periodical

770

Issue of the periodical within the volume

1

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

13

Pages from-to

261-273

UT code for WoS article

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EID of the result in the Scopus database

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