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Characterization of monomeric and oligomeric flavan-3-ols from barley and malt by liquid chromatography-ultraviolet detection-electrospray ionization mass spectrometry

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F08%3A00021106" target="_blank" >RIV/60461373:22330/08:00021106 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Characterization of monomeric and oligomeric flavan-3-ols from barley and malt by liquid chromatography-ultraviolet detection-electrospray ionization mass spectrometry

  • Original language description

    The pattern of the monomeric and oligomeric flavan-3-ols for 10 barley varieties and the corresponding malts were identified and quantified using high-performance liquid chromatography?ultraviolet detection?electrospray ion trap mass spectrometry. The Folin?Ciocalteau and the vanillin spectrophotometric assays were used for the assessment of the total polyphenol and total flavan-3-ol content, respectively, and the antioxidant activity was determined as the 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and the ferricyanide reducing power. Catechin and prodelphinidin B3 were respectively the major monomeric and dimeric flavan-3-ols. Moreover, prodelphinidin B3 was shown to be the main contributor for the radical scavenging activity both for barley and malt.

  • Czech name

    Charakterizace monomerních a oligomerních flavan-3-olů ječmene a sladu pomocí kapalinové chromatografie s UV a hmotnostní detekcí s elektrosprejovou ionizací

  • Czech description

    Pomocí vysokoúčinné kapalinové chromatografie s UV a hmotnostní detekcí (elektrosprej, iontová past) byly kvantifikovány monomerní a oligomerní flavan-3-oly v 10 odrůdách ječmene a odpovídajících sladech. The Folin?Ciocalteau a vanilinová spektrofotometrická metoda byly použity pro zhodnocení celkového obsahu polyfenolů a flavan-3-olů. Antioxidační aktivita byla stanovena metodou DPPH ( 2,2-diphenyl-1-picrylhydrazyl) a FRAP. Katechin byl hlavním monomerickým and prodelphinidin B3 byl hlavním dimerickýmflavan-3-olem. Mimo to, prodelphinidin B3 byl hlavní látkou s nejsilnější antioxidační kapacitou v ječmeni a sladu.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EI - Biotechnology and bionics

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/1M0570" target="_blank" >1M0570: Research Centre for Study of Extract Compounds of Barley and Hop</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2008

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Journal of Chromatography A

  • ISSN

    0021-9673

  • e-ISSN

  • Volume of the periodical

    1189

  • Issue of the periodical within the volume

    1-2

  • Country of publishing house

    NL - THE KINGDOM OF THE NETHERLANDS

  • Number of pages

    11

  • Pages from-to

  • UT code for WoS article

    000255698000040

  • EID of the result in the Scopus database