Batch and continuous hydrolysis of ovine whey proteins

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F08%3A00021224" target="_blank" >RIV/60461373:22330/08:00021224 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Batch and continuous hydrolysis of ovine whey proteins

Original language description

The aim of this work was to compare batch and continuous hydrolysis of ovine whey proteins in a membrane reactor and to evaluate influence of thermocalcic precipitation before hydrolysis. Reconstituted dried ovine whey fromlump cheese production (a traditional Slovak cheese) was used as a substrate for hydrolysis by Promod 439L protease from Bacillus licheniformis. Initial substrate had 2% proteins andpH8.5. Hydrolysis was performed directly (i) or after a pre-treatment (ii) comprising thermocalcic precipitation at 50 C followed by microfiltration at 40 C, batch-wise or in a membrane ultrafiltration (UF) reactor equipped with 8 kDa ceramic membrane. Mean residence time of 60 min was maintained after batch pre-hydrolysis of the substrate in the first 60min period, during which the permeate was recirculated. During the subsequent UF, the reaction volumewas kept constant by a continuous feeding of the substrate. After the pre-treatment (ii), crude protein decreased by 25%. Degree of hydr

Czech name

Vsádková a kontinuální hydrolýza proteinů ovčí syrovátky

Czech description

Cielom tejto práce bolo porovnanie vsádkovej a kontinuálnej hydrolýzy proteínov ovčej srvátky v membránovom reaktore a ohodnotiť vplyv termokalciovej precipitácie pred hydrolýzou. Obnovená sušená ovčia srvátka z výroby hrudky (tradičný slovenský syr) bola použitá ako substrát pre hydrolýzu s proteázou Promod 439L z Bacillus licheniformis.Východzí substrát obsahoval 2 % proteínov o ph 8.5. Hydrolýza bola robená buď priamo (i) alebo po ošetrení (ii) zahrňajúce termokalciovu precipitáciu pri 50C nasledovanou mikrofiltráciou pri 40C, vsádkovo alebo v membránovom ultrafiltračnom (UF) reaktore vybavenom s 8 kDa keramickou membránou. Doba zdržanie- 60 min bola zachovaná po vsádkovej predhydrolýze substrátu v prvých 60 min, v priebehu ktorých permeát recilkuroval. V priebehu následnej UF, reakčný objem bol držaný konštantne pomocou kontinuálne pridávaneho substrátu. Po ošetrní (ii), sa obsah proteínu znížil o 25%. Stupeň hydrolýzy bol 9.2% (i) a 8.8% (ii) po 60 min pri vsádkovej hydrolýze, a 1

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GM - Food industry

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2008

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Small Ruminant Research

ISSN

0921-4488

e-ISSN

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Volume of the periodical

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Issue of the periodical within the volume

79

Country of publishing house

BE - BELGIUM

Number of pages

6

Pages from-to

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UT code for WoS article

000259884800009

EID of the result in the Scopus database

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