Conformational changes of the N-terminal part of Mason-Pfizer monkey virus p12 protein during multimerization.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F09%3A00022119" target="_blank" >RIV/60461373:22330/09:00022119 - isvavai.cz</a>

Alternative codes found

RIV/61388971:_____/09:00341399

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Conformational changes of the N-terminal part of Mason-Pfizer monkey virus p12 protein during multimerization.

Original language description

The Mason-Pfizer monkey virus is a prototype Betaretrovirus with the defining characteristic that it assembles spherical immature particles from Gag-related polyprotein precursors within the cytoplasm of the infected cell. It was shown previously that the N-terminal part of the Gag p12 domain (wt-Np12) is required for efficient assembly. However, the precise role for p12 in mediating Gag-Gag interaction is still poorly understood. In this study we employed detailed circular dichroism spectroscopy, electron microscopy and ultracentrifugation analyses of recombinant wt-Np12 prepared by in vitro transcription and translation. The wt-Np12 domain fragment forms fibrillar structures in a concentration-dependent manner. Assembly into fibers is linked to a conformational transition from unfolded or another non-periodical state to alpha-helix during multimerization. (C) 2009 Elsevier Inc. All rights reserved.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Virology

ISSN

0042-6822

e-ISSN

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Volume of the periodical

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Issue of the periodical within the volume

393

Country of publishing house

BE - BELGIUM

Number of pages

9

Pages from-to

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UT code for WoS article

000270453600021

EID of the result in the Scopus database

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