Nisin-producing Lactococcus spp. from mayonnaise-based products and their raw materials

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F10%3A00024158" target="_blank" >RIV/60461373:22330/10:00024158 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Nisin-producing Lactococcus spp. from mayonnaise-based products and their raw materials
Original language description
Three bacteriocins were purified from lactic acid bacteria isolated from mayonnaise-based finished products and the raw materials used in the production of the finished products. All three bacteriocin-producing strains (EN3a, EN14a, and EN15b) were identified by sequencing of the 16S rDNA as Lactococcus lactis. The RP-HPLC fractions showed strong antimicrobial activity against Listeria monocytogenes. Mass spectrometry analysis of active fractions showed that all strains produced nisin A (3,353.7 Da). These results were confirmed by amplification of nisin structural gene by PCR, by lack of activity against a nisin-producing strain, and by total loss of activity after reaction of purified bacteriocins with glutathione. The strains Lactococcus lactis EN3a, EN14a, and EN15b are the first nisin-producing strains isolated from mayonnaise-based commercial products and from the raw materials used in their production. The ability of these strains to produce nisin could be useful to improve food
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GM - Food industry
OECD FORD branch
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Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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