Isolation of ?-lactoglobulin from whey by pepsin treatment.

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F10%3A00024388" target="_blank" >RIV/60461373:22330/10:00024388 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Isolation of ?-lactoglobulin from whey by pepsin treatment.
Original language description
?-Lactoglobulin (?-Lg) is the main protein of whey. It is highly resistant to pepsin digestion. We purified ?-Lg from fresh cheese whey by a combination of pepsin treatment and membrane filtration. Porcine pepsin (BC pepsin, 1:3000) was added to whey andthe mixture was then incubated at pH 3.5 and 40 °C for 180 minutes in the membrane unit ARNO 700 (Mikropur, s.r.o., Hradec Králové, Czech Republic). During hydrolysis, small peptides were removed using a tubular three channel ceramic TAMI-Industries ultrafiltration membrane (Hermsdorf, Germany) with the cut-off 50 kDa, pressure 0.350 MPa. After inactivation of the enzyme (pH adjusted to 7 by 10% NaOH), ultrafiltration retentate was divided into halves. The first one, was diafiltrated (DF) using the membrane with the cut-off 50 kDa. The second one, using the membrane with the cut-off 150 kDa. All samples, taken during filtrations and hydrolysis, were analyzed by RP-HPLC and the quantity was determined according to calibration. In both c
Czech name
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Czech description
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Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
Proceedings of the 37th International Conference of Slovak Society of Chemical Engineering, 2010
ISBN
978-80-227-3290-1
ISSN
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e-ISSN
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Number of pages
11
Pages from-to
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Publisher name
Slovak Society of Chemical Engineering
Place of publication
Bratislava
Event location
Tatranské Matliare, Slovakia
Event date
Jan 1, 2010
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
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