Disulfide bond decay during matrix-assisted laser desorption/ionization time-of-flight mass spectrometry experiments

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F11%3A43891997" target="_blank" >RIV/60461373:22330/11:43891997 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1002/rcm.5140" target="_blank" >http://dx.doi.org/10.1002/rcm.5140</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/rcm.5140" target="_blank" >10.1002/rcm.5140</a>

Result language
angličtina
Original language name
Disulfide bond decay during matrix-assisted laser desorption/ionization time-of-flight mass spectrometry experiments
Original language description
In our laboratory, we have been studying the reductive processes that occur during matrix-assisted laser desorption/ionization (MALDI) experiments. Recently, we have finished an analysis of the DHB matrix effect on the azo group in cyclic peptides. However, deep understanding of disulfide bond behaviour during a mass spectrometry (MS) experiment is much more important in proteomics as its reduction can cause serious errors in protein spectra interpretation. Therefore, we have focused on Intra- and intermolecular disulfide bonds as well as disulfide bonds connecting cysteine and 2-thio-5-nitrobenzoic acid (TNB, Ellman's reagent modification) in model peptides during MALDI MS measurements. While the reduction was not observed for Intra- and intermolecular cysteine-cysteine disulfide bonds, the disulfide connection between cysteine and TNB was always affected. It was proved that TNB and Ellman's reagent can act as a matrix itself. The results obtained enabled us to propose a reaction mech
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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