Analysis of isoflavones in soybeans employing direct analysis in real-time ionization-high-resolution mass spectrometry

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F12%3A43894790" target="_blank" >RIV/60461373:22330/12:43894790 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1002/jssc.201100882" target="_blank" >http://dx.doi.org/10.1002/jssc.201100882</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jssc.201100882" target="_blank" >10.1002/jssc.201100882</a>

Result language

angličtina

Original language name

Analysis of isoflavones in soybeans employing direct analysis in real-time ionization-high-resolution mass spectrometry

Original language description

A direct analysis in real-time (DART) ion source coupled to a high-resolution orbitrap mass spectrometer was used for the quantitative analysis of isoflavones isolated from soybeans. For the isolation of genistein, daidzein, glycitein, and their respective acetyl, malonyl, and glucoside forms an extraction employing 80% aqueous MeOH enhanced by sonication was used. As far as the total isoflavones (expressed as aglycones) were to be determined, an acid hydrolysis with 80% aqueous EtOH and refluxing had to be employed, while in the latter case a good agreement of the results with the data generated by the UHPLC-orbitrap MS method was achieved, in the case of the analysis of nonhydrolyzed extracts, some overestimation of the results as compared with thosegenerated by UHPLC-orbitrap MS was observed. A careful investigation of this phenomenon showed that the free aglycones originated from the conjugated forms of isoflavones in the DART ion source, thus contributing significantly to the ''f

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GM - Food industry

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Separation Science

ISSN

1615-9306

e-ISSN

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Volume of the periodical

35

Issue of the periodical within the volume

3

Country of publishing house

DE - GERMANY

Number of pages

6

Pages from-to

476-481

UT code for WoS article

000299254000018

EID of the result in the Scopus database

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