Simultaneous screening for T-2/HT-2 and deoxynivalenol in cereals using a surface plasmon resonance immunoassay
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F12%3A43894970" target="_blank" >RIV/60461373:22330/12:43894970 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.3920/WMJ2011.1351" target="_blank" >http://dx.doi.org/10.3920/WMJ2011.1351</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3920/WMJ2011.1351" target="_blank" >10.3920/WMJ2011.1351</a>
Alternative languages
Result language
angličtina
Original language name
Simultaneous screening for T-2/HT-2 and deoxynivalenol in cereals using a surface plasmon resonance immunoassay
Original language description
This manuscript describes a rapid surface plasmon resonance (SPR) immunoassay for the simultaneous determination of the sum of T-2/HT-2 toxins (T-2/HT-2) and deoxynivalenol (DON), in cereals and cereal-based products. The assay is based on an inhibitionformat employing a monoclonal antibody raised against HT-2 with cross reactivity to T-2 and a polyclonal antibody raised against DON, thereby enabling the detection of the three trichothecene mycotoxins (types A and B). The surface chemistry involved anequal mixture of HT-2 and DON covalently coupled onto a high capacity COONS sensor chip. Using the specified antibodies and a mixed toxin sensor surface, and running calibration curves (HT-2 and DON) and samples in parallel it has been proven that it isfeasible to develop a multiplex assay on this SPR platform. In-house validation has shown limits of detection of 12, 1 and 29 mu g/kg for DON and 31, 47 and 36 mu g/kg for HT-2 in wheat, breakfast cereal and maize-based baby food, respect
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GM - Food industry
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
WORLD MYCOTOXIN JOURNAL
ISSN
1875-0710
e-ISSN
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Volume of the periodical
5
Issue of the periodical within the volume
2
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
10
Pages from-to
117-126
UT code for WoS article
000304585700002
EID of the result in the Scopus database
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