Peptidoglycan Hydrolases as Species-Specific Markers to Differentiate Lactobacillus helveticus from Lactobacillus gallinarum and Other Closely Related Homofermentative Lactobacilli

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F14%3A43895925" target="_blank" >RIV/60461373:22330/14:43895925 - isvavai.cz</a>
Result on the web
<a href="http://link.springer.com/article/10.1007/s00284-013-0512-5" target="_blank" >http://link.springer.com/article/10.1007/s00284-013-0512-5</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00284-013-0512-5" target="_blank" >10.1007/s00284-013-0512-5</a>

Result language
angličtina
Original language name
Peptidoglycan Hydrolases as Species-Specific Markers to Differentiate Lactobacillus helveticus from Lactobacillus gallinarum and Other Closely Related Homofermentative Lactobacilli
Original language description
We propose a new method that allows accurate discrimination of Lactobacillus helveticus from other closely related homofermentative lactobacilli, especially Lactobacillus gallinarum. This method is based on the amplification by PCR of two peptidoglycan hydrolytic genes, Lhv_0190 and Lhv_0191. These genes are ubiquitous and show high homology at the intra-species level. The PCR method gave two specific PCR products, of 542 bp and 747 bp, for 25 L. helveticus strains coming from various sources. For L. gallinarum, two amplicons were obtained, the specific 542 bp amplicon and another one with a size greater than 1500 bp. No specific PCR products were obtained for 12 other closely related species of lactobacilli, including the Lactobacillus acidophilus complex, Lactobacillus delbrueckii, and Lactobacillus ultunensis. The developed PCR method provided rapid, precise and easy identification of L. helveticus. Moreover, it enabled differentiation between the two closely phylogenetically relate
Czech name
—
Czech description
—

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
—

Project
<a href="/en/project/QI101B090" target="_blank" >QI101B090: New processes of production of functional cereal and dairy foods and functional beverages with content of bioactive ingredients from selected vegetable and animal agricultural commodities and with probiotics and procedures for their quality estimation.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)