Characterization of transgenic tobacco plants containing bacterial bphC gene and study of their phytoremediation ability

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F14%3A43897358" target="_blank" >RIV/60461373:22330/14:43897358 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1080/15226514.2013.810575" target="_blank" >http://dx.doi.org/10.1080/15226514.2013.810575</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1080/15226514.2013.810575" target="_blank" >10.1080/15226514.2013.810575</a>

Result language
angličtina
Original language name
Characterization of transgenic tobacco plants containing bacterial bphC gene and study of their phytoremediation ability
Original language description
Genetically modified plants can serve as an efficient tool for remediation of diverse dangerous pollutants of the environment such as pesticides, heavy metals, explosives and persistent organic compounds. Transgenic lines of Nicotiana tabacum containingbacterial bphC gene from the degradation pathway of polychlorinated biphenyls (PCBs) were tested. The product of the bphC gene-enzyme 2,3-dihydroxybiphenyl-1,2-dioxygenase is responsible for cleaving of the biphenyl ring. The presence of bphC gene in transgenic plants was detected on DNA, RNA and protein level. The expression of the bphC/His gene was verified after purification of the enzyme from plants by affinity chromatography followed by a Western blot and immunochemical assay. The enzyme activity of isolated protein was detected. Efficient transformation of 2,3-DHB by transgenic plants was achieved and the lines also exhibited high production of biomass. The transgenic plants were more tolerant to the commercial PCBs mixture Delor
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Project
<a href="/en/project/LH12087" target="_blank" >LH12087: Microbial diversity and plant-microbe interactions of contaminant-remediating plants ? Optimizing symbiotic synergism for phytoremediation</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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