Direct evidence for intracellular anterograde co-transport of M-PMV Gag and Env on microtubules
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F14%3A43897526" target="_blank" >RIV/60461373:22330/14:43897526 - isvavai.cz</a>
Result on the web
<a href="http://www.sciencedirect.com/science/article/pii/S0042682213006193" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0042682213006193</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.virol.2013.11.006" target="_blank" >10.1016/j.virol.2013.11.006</a>
Alternative languages
Result language
angličtina
Original language name
Direct evidence for intracellular anterograde co-transport of M-PMV Gag and Env on microtubules
Original language description
The intracellular transport of Mason-Pfizer monkey virus (M-PMV) assembled capsids from the pericentriolar region to the plasma membrane (PM) requires trafficking of envelope glycoprotein (Env) to the assembly site via the recycling endosome. However, itis unclear if Env-containing vesicles play a direct role in trafficking capsids to the PM. Using live cell microscopy, we demonstrate, for the first time, anterograde co-transport of Gag and Env. Nocodazole disruption of microtubules had differential effects on Gag and Env trafficking, with pulse-chase assays showing a delayed release of Env-deficient virions. Particle tracking demonstrated an initial loss of linear movement of GFP-tagged capsids and mCherry-tagged Env, followed by renewed movement ofGag but not Env at 4 h post-treatment. Thus, while delayed capsid trafficking can occur in the absence of microtubules, efficient anterograde transport of capsids appears to be mediated by microtubule-associated Env-containing vesicles.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Virology
ISSN
0042-6822
e-ISSN
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Volume of the periodical
449
Issue of the periodical within the volume
leden 2014
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
109-119
UT code for WoS article
000330094100013
EID of the result in the Scopus database
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