Structure of the immature HIV-1 capsid in intact virus particles at 8.8Ao resolution

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F15%3A43897365" target="_blank" >RIV/60461373:22330/15:43897365 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1038/nature13838" target="_blank" >http://dx.doi.org/10.1038/nature13838</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/nature13838" target="_blank" >10.1038/nature13838</a>

Result language

angličtina

Original language name

Structure of the immature HIV-1 capsid in intact virus particles at 8.8Ao resolution

Original language description

Human immunodeficiency virus type 1 (HIV-1) assembly proceeds in two stages. First, the 55 kilodalton viral Gag polyprotein assembles into a hexameric protein lattice at the plasma membrane of the infected cell, inducing budding and release of an immature particle. Second, Gag is cleaved by the viral protease, leading to internal rearrangement of the virus into the mature, infectious form1. Immature and mature HIV-1 particles are heterogeneous in size andmorphology, preventing high-resolution analysis of their protein arrangement in situ by conventional structural biology methods. Here we apply cryo-electron tomography and sub-tomogram averaging methods to resolve the structure of the capsid lattice within intact immature HIV-1 particles at subnanometre resolution, allowing unambiguous positioning of all a-helices. The resulting model reveals tertiary and quaternary structural interactions that mediate HIV-1 assembly. Strikingly, these interactions differ from those predicted by the cu

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EI - Biotechnology and bionics

OECD FORD branch

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Project

<a href="/en/project/GA14-15326S" target="_blank" >GA14-15326S: Study of structure and interactions critical for formation of immature retroviral particles for testing and rational design of HIV assembly inhibitors</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nature

ISSN

0028-0836

e-ISSN

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Volume of the periodical

517

Issue of the periodical within the volume

leden

Country of publishing house

GB - UNITED KINGDOM

Number of pages

4

Pages from-to

505-508

UT code for WoS article

000348196500039

EID of the result in the Scopus database

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