Two different methods for screening of bile salt hydrolase activity in Lactobacillus strains
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F15%3A43899624" target="_blank" >RIV/60461373:22330/15:43899624 - isvavai.cz</a>
Result on the web
<a href="http://www.agriculturejournals.cz/web/cjfs.htm?volume=33&type=volume#No" target="_blank" >http://www.agriculturejournals.cz/web/cjfs.htm?volume=33&type=volume#No</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.17221/299/2014-CJFS" target="_blank" >10.17221/299/2014-CJFS</a>
Alternative languages
Result language
angličtina
Original language name
Two different methods for screening of bile salt hydrolase activity in Lactobacillus strains
Original language description
Bile salt hydrolase (BSH) activity of intestinal bacteria (including lactobacilli) is one of indirect ways of decreasing cholesterol level at human body. Tested Lactobacillus strains were isolated from various sources (faeces of fully breast-fed infants,cow's colostrum, raw cow's milk cheeses and raw cow's milk) and identified by genotypic and phenotypic methods. All strains, including two commercial probiotic strains and five collection strains, were subsequently tested for their BSH activity via twomethods - Thin Layer Chromatography (TLC) and plate assay. Among all the 45 Lactobacillus strains, 11 strains were shown to be BSH positive by TLC method and only 5 of them by plate assay. Most of the BSH positive strains (7 strains) were isolated from faeces. Differences between BSH activities for sodium salts of cholyltaurine and cholylglycine were demonstrated. TLC method was shown to be more sensitive compared to plate assay for BSH activity determination.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GM - Food industry
OECD FORD branch
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Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Czech Journal of Food Sciences
ISSN
1212-1800
e-ISSN
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Volume of the periodical
33
Issue of the periodical within the volume
1
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
6
Pages from-to
13-18
UT code for WoS article
000351653300003
EID of the result in the Scopus database
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