Separation of immunoglobulins from colostrum using methods based on salting out techniques.

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F17%3A43914812" target="_blank" >RIV/60461373:22330/17:43914812 - isvavai.cz</a>
Alternative codes found
RIV/28676092:_____/17:N0000064
Result on the web
<a href="http://www.agriculturejournals.cz/web/cjfs.htm?volume=35&firstPage=259&type=publishedArticle" target="_blank" >http://www.agriculturejournals.cz/web/cjfs.htm?volume=35&firstPage=259&type=publishedArticle</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.17221/10/2017-CJFS" target="_blank" >10.17221/10/2017-CJFS</a>

Result language
angličtina
Original language name
Separation of immunoglobulins from colostrum using methods based on salting out techniques.
Original language description
Immunoglobulins (Igs) are one of the most important proteins in the organisms of animals. The highest concentration of Igs (predominantly IgG) in nature is detected in colostrum. This study aimed to obtain a purified IgG fraction from bovine colostrum using salting out precipitation methods as base techniques. As the first step after skimming of colostrum, isoelectric precipitation or rennet coagulation were used for casein removal. IgG concentrations in raw material and final products were determined using ELISA method. SDS-PAGE electrophoresis was used for purity determination of the final fraction of IgG. According to the obtained results, combination of precipitation, gel filtration and cross-flow filtration techniques enables to separate IgG fraction with purity up to 91% and yield up to 86.3%. Acid whey has higher yield in comparison to sweet whey and both ammonium and sodium sulfate have similar effectivity for precipitation of IgG. Final concentrate of IgG can be used as a standalone product or as additive for other food supplements.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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