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Rapid detection of microbial contamination in UHT milk: Practical application in dairy industry

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F18%3A43917322" target="_blank" >RIV/60461373:22330/18:43917322 - isvavai.cz</a>

  • Alternative codes found

    RIV/60461373:22810/18:43917322

  • Result on the web

    <a href="https://www.agriculturejournals.cz/web/cjfs.htm?type=article&id=24_2018-CJFS" target="_blank" >https://www.agriculturejournals.cz/web/cjfs.htm?type=article&id=24_2018-CJFS</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.17221/24/2018-CJFS" target="_blank" >10.17221/24/2018-CJFS</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Rapid detection of microbial contamination in UHT milk: Practical application in dairy industry

  • Original language description

    Microbial quality of ultra-high temperature (UHT) milk is usually ascertained by a total bacterial count (TBC) cultivation. But this is time consuming, so there is a tendency to search for faster and simpler methods. We compared three instruments, focusing on shortening the detection time and their suitability for practical use in dairy plants. Two of them, RapiScreen Dairy 1000 and Promilite III, detect microbial contamination by measuring adenosine triphosphate bioluminescence; the third, GreenLight, is based on oxygen consumption analysis. In the laboratory experiments, samples of UHT milk, were spiked with low concentration of microorganisms and then the level of microbial contamination was evaluated using the above-mentioned instruments together with cultivation method as a control. The instruments were also applied in a dairy plant to test 182 real samples. All investigated methods determined microbial quality faster than the TBC, but in some cases false positive and false negative results were obtained. Therefore, precise testing including optimizing pre-incubation time for bacteria enrichment is needed prior to industrial use.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Result continuities

  • Project

  • Continuities

    S - Specificky vyzkum na vysokych skolach

Others

  • Publication year

    2018

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Czech Journal of Food Science

  • ISSN

    0862-8653

  • e-ISSN

  • Volume of the periodical

    36

  • Issue of the periodical within the volume

    5

  • Country of publishing house

    CZ - CZECH REPUBLIC

  • Number of pages

    8

  • Pages from-to

    357-364

  • UT code for WoS article

    000451238600001

  • EID of the result in the Scopus database