Optimisation of the method for determination of B-complex vitamins in infant formula
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F19%3A43919076" target="_blank" >RIV/60461373:22330/19:43919076 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Optimisation of the method for determination of B-complex vitamins in infant formula
Original language description
B-complex vitamins (thiamine (B1), riboflavin (B2), niacin (B3), pantothenic acid (B5), pyridoxine (B6), biotin (B7), folic acid (B9), cobalamin (B12)) represent a group of chemical individuals with different structure, stability, solubility in different solutions and mainly occur in a various amount in food commodities. These hydrophilic vitamins can also occur in various form as a free or bound on proteins, phosphorylated beside that some of them must be quantified as a sum of several substances (e.g. B9 (folic acid and 5-methyl tetrahydrofolate or B6 (pyridoxal, pyridoxine and pyridoxamine)). All these factors should be taken into account during choosing an appropriate analytical method for both isolation and determination of these compounds in food samples including infant formula. The aim of this study was to optimize and validate a method for simultaneous determination of 8 hydrophilic vitamins and their forms in infant formula. Two different sample preparation approaches were tested (with and without enzymatic hydrolysis). For the purification of the obtained extracts solid phase extraction (SPE) was employed. Identification and quantification of target analytes was performed using high performance liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). The performance characteristics of the validated method obtained by the analysis of the SRM 1849a (infant/adult nutritional formula, milk-based) in six replicates, for the procedure without the hydrolysis step, were as follows the recovery 42-103 % (for nine analytes) with repeatability 4-15 % and the limits of quantification 10-25 ng/ml. For three of the target analytes (B1, B9 and B12) the method was not validated.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/LM2018100" target="_blank" >LM2018100: Infrastructure for Promoting Metrology in Food and Nutrition in the Czech Republic</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů