Selective culture medium for the enumeration of Lactobacillus plantarum in the presence of Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F19%3A43919286" target="_blank" >RIV/60461373:22330/19:43919286 - isvavai.cz</a>

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S0023643819307078?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0023643819307078?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.lwt.2019.108365" target="_blank" >10.1016/j.lwt.2019.108365</a>

Result language

angličtina

Original language name

Selective culture medium for the enumeration of Lactobacillus plantarum in the presence of Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus

Original language description

Selective enumeration of Lactobacillus plantarum in a mixed culture with yogurt bacteria is difficult, due to their similar growth conditions. We evaluated several culture media to determine their suitability for selective determination. The following media were tested to enumerate the strains of Lactobacillus plantarum, Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus: M17, MRS pH 5.2; 5.6 and 6.2, and modified MRS where glucose was replaced by maltose, sorbitol, mannitol, sucrose or galactose. Further, the addition of vancomycin (20 mg/l) and the use of different cultivation conditions - aerobic or anaerobic, and different cultivation temperatures 30 degrees C, 37 degrees C and 45 degrees C, were chosen to improve selectivity. MRS medium with vancomycin (pH 5.6), and cultivation under aerobic conditions at 30 degrees C was successfully used for selective enumeration of L. plantarum in a mixed culture with yogurt bacteria after cultivation both in broth and in milk. To distinguish between the remaining bacteria, M17 medium under aerobic conditions at 45 degrees C and MRS pH 5.2 under anaerobic conditions under 45 degrees C were recommended for counting of S. thermophilus and L. delbrueckii subsp. bulgaricus, respectively. No statistical differences were found between pour plate and surface plate methods of inoculation (P &gt; 0.05).

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

21101 - Food and beverages

Project

<a href="/en/project/QK1910036" target="_blank" >QK1910036: Utilization of various metabolites of wild strains of lactic acid bacteria and yeasts to suppress the occurrence of undesirable fungal contaminants and to prolong the shelf life of dairy and bakery products.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

LWT - Food Science and Technology

ISSN

0023-6438

e-ISSN

—

Volume of the periodical

114

Issue of the periodical within the volume

November 2019, 108365

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

7

Pages from-to

1-7

UT code for WoS article

000482245900005

EID of the result in the Scopus database

2-s2.0-85068502085