Enzymatic synthesis of galactooligasaccharides using commercial β-galactosidases

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F19%3A43919914" target="_blank" >RIV/60461373:22330/19:43919914 - isvavai.cz</a>
Alternative codes found
RIV/60461373:22330/19:43919916
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Enzymatic synthesis of galactooligasaccharides using commercial β-galactosidases
Original language description
Galactooligosaccharides (GOS) are widely used in the functional food industry due health-promoting effects as a prebiotic in humans. GOS can be synthesized by transgalactosylation reactions from lactose using β-galactosidase. In this study we investigate enzymatic activity on GOS formation of the next commercial enzymes: Maxilact LGI 5000, Maxilact A4 MG and two new enzymes - NOLA Fit 5500 and Saphera 2600 L. The aim of the present work was to gain the highest yield of galactooligosaccharides using commercial β-galactosidases and reduced concentration of residual lactose. The experiments were held at 40°C with initial lactose concentration of 500-550 g∙L-1 in phosphate buffer. Qualitative and quantitative data were acquired using HPLC and HPAEC-PAD. High concentration of GOS with low lactose content in solution increases the efficiency of galactooligosaccharides purification that could be potentially used in the further investigations.
Czech name
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Czech description
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Project
<a href="/en/project/QK1910024" target="_blank" >QK1910024: Utilization of enzyme and probiotics potential of micro-organisms to develop new and improve the quality and durability of existing dairy and bakery products.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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