Fish species identification by pcr using parvalbumin gene as a platform

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F19%3A43920124" target="_blank" >RIV/60461373:22330/19:43920124 - isvavai.cz</a>

Alternative codes found

RIV/00027022:_____/20:999

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Fish species identification by pcr using parvalbumin gene as a platform

Original language description

Food fraud is a significant and growing problem. The increase in international trade, increasing global consumption of fish and different level of supplies and demands for some species, have led to many cases of economic frauds. In this case one type of fish product is illegally replaced by another. This is big economical problem, because mislabeling can result as fraudulent substitution of meat with high value with some less expensive fish. Moreover, proper labeling is also important in terms of the impact on health, certain people can consume only specific fishes, because of allergic sensitivity. The most common determination of fish species is based on morphological traits. This approach faces more and more complications as the level of processing fish flesh into products of food industry and/or complex dishes in gastronomy makes morphological markers less available. Methods based on the polymerase chain reaction (PCR) are the most spread for control purposes, due to the high level of sensitivity and specificity. The presented analysis is performed as an amplification of nuclear gene encoding important protein of fish muscles parvalbumin, also known as a major fish allergen. Conventional PCR method for the differentiation of the following fish species were developed: black seabream (Sponyliosoma cantharus), Atlantic mackerel (Scomber scombrus), iridescent shark (Pangasianodon hypophthalmus) and angler fish (Lophius piscatorius). Specificity was verified on panel of 19 different fish species. This method can be employed as a routine tool for species origin determination irrespectively of morphological traits.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

40103 - Fishery

Project

<a href="/en/project/QK1910231" target="_blank" >QK1910231: New approaches for the proof of fish meat adulteration using genomic DNA</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů