PCR-based fish meat authentication

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027022%3A_____%2F17%3A00000677" target="_blank" >RIV/00027022:_____/17:00000677 - isvavai.cz</a>

Result on the web

<a href="http://www.crf2017.eu/crf_2017_BoA.html" target="_blank" >http://www.crf2017.eu/crf_2017_BoA.html</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

PCR-based fish meat authentication

Original language description

The most common determination of fish species is based on morphological traits. This approach faces more and more complications as the level of processing of fish flesh into products of food industry and/or complex dishes produced in gastronomy makes morphological markers less available. Another factor preventing morphological determination is the character of marine fish trade and import of this fish into landlocked countries like the Czech Republic. These are often transported in the form of frozen blocks, also devoid of morphological characteristics. It is therefore critical to have a method for species determination independent on size, intactness or level of processing of the sample, available as a versatile tool. Towards this end, methods of molecular biology based on Polymerase Chain Reaction (PCR) appear as the best tool due to their high level of sensitivity and specificity in raw as well as technologically processed fish meat, even in the form of complex food products. The presented analysis is performed as an amplification of nuclear gene encoding important protein of fish muscles parvalbumin, also known as a major allergen connected with fish consumption. Here, within the scope of Real-Time PCR (qPCR) set of primers together with fluorescent dye-conjugated probe, both specific to second intron of protein coding region of Black seabream (Spondyliosoma cantharus) were designed and tested. Specificity of this set was validated on panel of 19 different fish species representing the most common and important fraction of the spectrum of the locally traded species, i.e. for instance Carp (Cyprinus carpio), Tench (Tinca tinca), Nile tilapia (Oreochromis niloticus) and others. Efficiency of the method was determined from calibration curve based on points of five levels of DNA concentration. Quantitative PCR presented here was proven as highly efficient and specific method for Black seabream (S. cantharus) species identification.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů