Determination of carotenoids in flowers and food supplements by HPLC-DAD

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F20%3A43921061" target="_blank" >RIV/60461373:22330/20:43921061 - isvavai.cz</a>

Result on the web

<a href="https://content.sciendo.com/configurable/contentpage/journals$002facs$002f13$002f1$002farticle-p6.xml" target="_blank" >https://content.sciendo.com/configurable/contentpage/journals$002facs$002f13$002f1$002farticle-p6.xml</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2478/acs-2020-0002" target="_blank" >10.2478/acs-2020-0002</a>

Result language

angličtina

Original language name

Determination of carotenoids in flowers and food supplements by HPLC-DAD

Original language description

Marigold flowers (Tagetes patula and Calendula officinalis) were chosen for analysis because they are the most often used source of lutein and its isomer zeaxanthin for the production of food supplements on the Czech market. Direct extraction and extraction with alkaline hydrolysis were compared to detect free or bound carotenoids. For carotenoid separation, C18 and C30 columns were used. A new method for determination of cal oienoid content in food supplements in form of capsules has been developed and validated. All matrices were analysed by high-pet lot malice liquid chromatography with diode array detection (HPLC-DAD). It has been found that alkaline hydrolysis is required for both Marigold flowers and food supplements to release lutein from ester bonds to fatty acids. In Calendula officin,alislutein in the concentration of 807-1472 mg . kg(-1) of dry matter was detected. Tagetes patula has been identified as a better lutein source with the content of 5906-8677 mg . kg(-1) of dry matter. It has been found that the content of lutein and zeaxanthin in commercial food supplements (Lutein Complex Premium and Occutein Brillant) is consistent with the declared quantity. Linearity of the HPI.C-DAD method ranged from 0.1-20 mu g . mL(-1) with the limit of quantification (LOQ) of 1.7 mg . kg(-1) for lutein in Marigold flowers and 200 mg . kg(-1) in food supplements. Repeatability was 2.3 % for lutein in all tested matrices.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10406 - Analytical chemistry

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Chimica Slovaca

ISSN

1337-978X

e-ISSN

—

Volume of the periodical

13

Issue of the periodical within the volume

1

Country of publishing house

PL - POLAND

Number of pages

7

Pages from-to

6-12

UT code for WoS article

000546283900002

EID of the result in the Scopus database

—