Identification of homologous polyprenols from thermophilic bacteria

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F21%3A43922991" target="_blank" >RIV/60461373:22330/21:43922991 - isvavai.cz</a>

Alternative codes found

RIV/61388971:_____/21:00546635 RIV/60193697:_____/21:N0000019

Result on the web

<a href="https://www.mdpi.com/2076-2607/9/6/1168/htm" target="_blank" >https://www.mdpi.com/2076-2607/9/6/1168/htm</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/microorganisms9061168" target="_blank" >10.3390/microorganisms9061168</a>

Result language

angličtina

Original language name

Identification of homologous polyprenols from thermophilic bacteria

Original language description

Sixteen strains of five genera of thermophilic bacteria, i.e., Alicyclobacillus, Brevibacillus, Geobacillus, Meiothermus, and Thermus, were cultivated at a temperature from 42 to 70 °C. Twelve strains were obtained from the Czech Collection of Microorganisms, while four were directly isolated and identified by 16S rRNA gene sequencing from the hot springs of the world‐famous Carlsbad spa (Czech Republic). Polyprenol homologs from C40 to C65 as well as free undecaprenol (C55), undecaprenyl phosphate, and undecaprenyl diphosphate were identified by shotgun analysis and RP‐HPLC/MS‐ESI+ (reverse phase high‐performance liquid chromatography–high‐resolution positive electrospray ionization mass spectrometry). The limit of detection (50 pM) was determined for individual homologs and free polyprenols and their phosphates. Thus, it has been shown that at least some thermophilic bacteria produce not just the major C55 polyprenol as previously described, but a mixture of homologs. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

20801 - Environmental biotechnology

Project

<a href="/en/project/GA18-00036S" target="_blank" >GA18-00036S: Ecology of extremophilic microorganisms in Czech spring waters of cultural heritage significance</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Microorganisms

ISSN

2076-2607

e-ISSN

—

Volume of the periodical

9

Issue of the periodical within the volume

6

Country of publishing house

CH - SWITZERLAND

Number of pages

12

Pages from-to

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UT code for WoS article

000666057600001

EID of the result in the Scopus database

2-s2.0-85106602333