Off-line two-dimensional LC–tandem MS of menaquinones from thermophilic bacteria

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F23%3A43927129" target="_blank" >RIV/60461373:22330/23:43927129 - isvavai.cz</a>

Alternative codes found

RIV/60193697:_____/24:N0000011 RIV/61388971:_____/24:00579397

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S0308814623017302#s0085" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0308814623017302#s0085</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.foodchem.2023.137112" target="_blank" >10.1016/j.foodchem.2023.137112</a>

Result language

angličtina

Original language name

Off-line two-dimensional LC–tandem MS of menaquinones from thermophilic bacteria

Original language description

Thermophilic bacteria of four genera in contrast to the commonly used production strains such as Bacillus subtilis, produce homologs other than menaquinone (MK) with seven isoprene units. The number of isoprene units and the configuration of double bonds are essential factors for their biological activity. The goal was to obtain a strain of bacteria that produces a wide range of MK homologs and only all-trans geometrical isomers, which was the strain G. kaustophilus. Using off-line two-dimensional LC–tandem MS in columns with the RP18 phase and the COSMOSIL cholester phase (separation according to the geometric configuration of double bonds) it was shown that thermophilic bacteria grown at different temperatures produce only all-trans isomers of menaquinones from MK-5 (menaquinone with five isoprenyl units) to MK-15 (fifteen isoprenyl units). Therefore, G. kaustophilus appears to be a biotechnologically important strain produces only trans isomers and additionally homologs from 5 to 15 isoprene units.

Czech name

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Czech description

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Type

J_ost - Miscellaneous article in a specialist periodical

CEP classification

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OECD FORD branch

10406 - Analytical chemistry

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Food Chemistry

ISSN

0308-8146

e-ISSN

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Volume of the periodical

431

Issue of the periodical within the volume

1

Country of publishing house

BE - BELGIUM

Number of pages

8

Pages from-to

1-8

UT code for WoS article

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EID of the result in the Scopus database

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