Bioprospecting of Artemisia scoparia, Panax ginseng and Phellodendron sp.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F23%3A43928063" target="_blank" >RIV/60461373:22330/23:43928063 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Bioprospecting of Artemisia scoparia, Panax ginseng and Phellodendron sp.

Original language description

Many herbal extracts are known to contain biologically active molecules, some of which find uses in pharmaceuticals production. In the recent years, substances with a potential to inhibit lipolysis are being searched. One of potential applications is a treatment of obstructive sleep apnea, chronic disease which causes hypoxia and disrupts adipocyte metabolism. The aim of our study was to isolate and identify novel bioactive secondary metabolites from Artemisia scoparia, Panax ginseng and Phellodendron sp., which are presumed to contain lipolysis inhibitors. To obtain the entire set of compounds with different polarities, three types of extracts were prepared from each dried plant: aqueous, methanolic and dichloromethane extracts. These extracts were then fractionated using high performance liquid chromatography Waters AutoPurification System coupled to single quadrupole mass spectrometry detector (QDa). Nine sets of fractions were collected and their antilipolytic activity was measured. An enzymatic assay based on measurement of released free glycerol concentration was employed both for spontaneous and isoprenaline-induced lipolysis. Significant fractions with the highest inhibitory activity were further analyzed using ultra high-performance liquid chromatography coupled with tandem high resolution mass spectrometry, in order to identify compounds responsible for respective bioactivity.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

<a href="/en/project/NU21-01-00259" target="_blank" >NU21-01-00259: Novel inhibitors of lipolysis in the treatment of lipid a glucose metabolism in obstructive sleep apnea syndrome</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů