Assessing metabolic activity of yeast biofilm-forming cells on nanofibrous materials using MTT assay

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F25%3A43932231" target="_blank" >RIV/60461373:22330/25:43932231 - isvavai.cz</a>

Result on the web

<a href="https://link.springer.com/article/10.1007/s12223-025-01304-1" target="_blank" >https://link.springer.com/article/10.1007/s12223-025-01304-1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s12223-025-01304-1" target="_blank" >10.1007/s12223-025-01304-1</a>

Result language

angličtina

Original language name

Assessing metabolic activity of yeast biofilm-forming cells on nanofibrous materials using MTT assay

Original language description

Nanofibrous materials (NMs), widely used in medical and food industry applications, are highly susceptible to colonisation by microorganisms, including yeasts. Although yeasts can form dense biofilms, methods for studying their metabolic activity remain limited. This study is the first to apply the MTT assay, a standardised method for assessing cell metabolic activity, to evaluate the metabolic activity of yeast biofilm-forming cells on electrospun NMs. First, the biofilm formation of Candida albicans and Saccharomyces cerevisiae on NMs electrospun from polycaprolactone (PCL), polylactic acid (PLA), and polyamide (PA) was examined. Then, key parameters of the MTT assay were systematically evaluated: (i) the addition of glucose to the MTT solution, (ii) the presence of menadione in the MTT solution, and (iii) the incubation time with the MTT solution. The addition of glucose was not proven necessary; however, in some cases, it may help distinguish the number of metabolically active cells. Based on this study, we recommend incubation with an MTT solution containing menadione for 2 h. To verify the protocol, colony-forming unit (CFU) enumeration was employed as a reference method. As differences between the results of these two methods were observed, the MTT assay should be complemented by other standardised methods. Nevertheless, the refined protocol offers a solid basis for investigating interactions between NMs and yeasts. © The Author(s) 2025.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10612 - Mycology

Project

<a href="/en/project/GA23-05154S" target="_blank" >GA23-05154S: Investigation of prokaryotic and eukaryotic cell interactions with nanofibers differing in morphology and structure</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

FOLIA MICROBIOLOGICA

ISSN

0015-5632

e-ISSN

—

Volume of the periodical

neuveden

Issue of the periodical within the volume

July 2025

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

9

Pages from-to

nestránkováno

UT code for WoS article

001537751700001

EID of the result in the Scopus database

2-s2.0-105012182970