Quantification of copper and zinc species fractions in legume seeds extracts by SEC/ICP-MS: validation and uncertainty estimation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F02%3A00007187" target="_blank" >RIV/60461373:22340/02:00007187 - isvavai.cz</a>

Alternative codes found

RIV/60461373:22330/02:00007187

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Quantification of copper and zinc species fractions in legume seeds extracts by SEC/ICP-MS: validation and uncertainty estimation

Original language description

Fractions of Cu and Zn species in legume samples (common white bean, pea, chick pea and lentil seeds and defatted soybean flour) were analyzed by on-line hyphenation of size exclusion chromatography and inductively coupled plasma-mass spectrometry. Samples were extracted by 0.02 mol/L Tris-HCl buffer solution, pH = 7.5. The extraction efficiency lay in the region 60 - 90 % and 60 - 80 % for Cu and Zn respectively. Quantification of elements in the individual chromatographic fractions was carried out byisotope dilution (ID) and external calibration (EC) techniques. For ID analysis the chromatographic effluent was mixed with the flow of 65Cu and 68Zn isotope enriched solution and the isotope ratio values 63Cu/65Cu and (64Zn+66Zn)/68Zn were measured. Inthe case of EC technique calibration solutions of elements were injected to the flow of mobile phase by the second injector. Prior entering detector the effluent was mixed with the flow of internal standard solution (In, 50 g/L). Both met

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

<a href="/en/project/GA525%2F00%2F0581" target="_blank" >GA525/00/0581: Speciation analysis of trace elements in foods</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2002

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Talanta

ISSN

0039-9140

e-ISSN

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Volume of the periodical

57

Issue of the periodical within the volume

6

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

12

Pages from-to

1133-1142

UT code for WoS article

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EID of the result in the Scopus database

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