Application of size exclusion chromatography - inductively coupled plasma - mass spectrometry for fractionation of element species in seeds of legumes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F02%3A00007174" target="_blank" >RIV/60461373:22340/02:00007174 - isvavai.cz</a>

Alternative codes found

RIV/60461373:22330/02:00007174

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Application of size exclusion chromatography - inductively coupled plasma - mass spectrometry for fractionation of element species in seeds of legumes

Original language description

Fractionation of soluble species of P, Mn, Fe, Co, Ni, Cu, Zn, Se and Mo in pea and lentil seeds was made by on-line hyphenation of SEC and ICP-MS. Seed samples were extracted by 0.02 mol l-1 Tris-HCl buffer solution, pH=7.5. SEC was performed on Superdex 75 and Superdex Peptide columns (300 10 mm) with the same buffer solution as the mobile phase. Monitoring of oxide ion 47(PO)+ was used for detection of phosphorus compounds. Other elements were detected as ions of 55Mn, 57Fe, 59Co, 62Ni, 65Cu, 66Zn, 82Se and 95Mo nuclides. Elements in individual elution zones were quantified using external calibration. Complete chromatographic recoveries of elements were found in cases of phosphorus, nickel and copper. Substantial parts of manganese and zinc, as wellas traces of cobalt, selenium and molybdenum are retained on the column. Injection of EDTA solution removes these elements from the column. Chromatographic profiles of pea and lentil samples are very similar for all elements except Mo. Ma

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

<a href="/en/project/GA525%2F00%2F0581" target="_blank" >GA525/00/0581: Speciation analysis of trace elements in foods</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2002

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Chromatography B

ISSN

1570-0232

e-ISSN

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Volume of the periodical

778

Issue of the periodical within the volume

1

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

9

Pages from-to

179-187

UT code for WoS article

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EID of the result in the Scopus database

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