Novel porphyrin conjugates with a potent photodynamic antitumor effect: differential efficacy of mono- and bis-b-cyclodextrin derivatives. In vitro and in vivo

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F06%3A00016680" target="_blank" >RIV/60461373:22340/06:00016680 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Novel porphyrin conjugates with a potent photodynamic antitumor effect: differential efficacy of mono- and bis-b-cyclodextrin derivatives. In vitro and in vivo

Original language description

In the present study we investigated photosensitizing properties of two novel mono- and bis-cyclodextrin tetrakis(pentafluorophenyl) porphyrin derivatives in several tumor cell lines and in BALB/c mice bearing subcutaneously transplanted syngeneic mousemammary carcinoma, 4T1. Both studied sensitizers were localized mainly into lysosomes and were found to induce cells death by triggering apoptosis in human leukemic cells HL-60. In 4T1 and other cell lines both apoptotic and necrotic modes of cell deathoccurred dependently on drug and light doses. Mono-cyclodextrin porphyrin derivative, P(b-CD)1, exhibited stronger in vitro photodynamic efficacy than bis-cyclodextrin derivative, P(b-CD)2. However, when their efficacy was evaluated in vivo, P(b-CD)2 displayed faster tumor uptake with maximal accumulation 6 h after application leading to complete and prolonged elimination of subcutaneous tumors within 3 days after irradiation (100 J/cm2). In contrast P(b-CD)1 uptake was slower (48 h) and

Czech name

Nové porfyrinové conjugaty s fotodynamickým protinádorovým efektem: účinnost mono- a bis-b-cyclodextrinových derivátů v in vivo a in vitro studiu

Czech description

V představeném studiu byly sledované fotosensibilní vlastnosti dvou nových mono- a bis-cyclodextrin tetrakis(pentafluorofenyl) porfyrinových derivátů na kulturách nádorových buněk a na nádoru prsní žlázy 4T1 transplantovaného pod kůži BALB/c myši. Obě studované látky v experimentech in vitro prokázaly lokalizace v lysosomech buněčných kultur a indukovaly smrt buněk spouštěním apoptosy. Mono-cyclodextrin porfyrinový derivát, P(b-CD)1, projevoval silnější fotodynamický efekt in vitro než bis-cyclodextrinový derivat P(b-CD)2. Ale v experimentech in vivo P(b-CD)2 ukázal rychlejší lokalizace v nádorové tkáni s maximální obsahem už za 6 hodin po aplikace a silnější protinádorovou aktivitu na rozdíl od P(b-CD)1 z pomalejší lokalizace.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

<a href="/en/project/GA301%2F04%2F1315" target="_blank" >GA301/04/1315: Induction of apoptosis by macrocyclic compounds in cancer cells</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2006

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Photochemistry and Photobiology

ISSN

0031-8655

e-ISSN

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Volume of the periodical

82

Issue of the periodical within the volume

2

Country of publishing house

CA - CANADA

Number of pages

7

Pages from-to

432-438

UT code for WoS article

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EID of the result in the Scopus database

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