Microfluidic chip for fast bioassays-evaluation of binding parameters
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F07%3A00018089" target="_blank" >RIV/60461373:22340/07:00018089 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Microfluidic chip for fast bioassays-evaluation of binding parameters
Original language description
A seven channel polystyrene (PS) microchip has been constructed using a micromilling machine and a high-temperature assembling. Protein A (PA) has been immobilized by a passive sorption on the microchannel walls. Two bioaffinity assays with human immunoglobulin G (hIgG) as a ligand have been carried out. (i) PA as the receptor and fluorescently labeled hIgG (FITC-hIgG) as the ligand, (ii) PA as the receptor with hIgG as the quantified ligand and fluorescently labeled goat anti-human IgG (FITC-gIgG) as the secondary ligand. One incubation step of the assays took only 5 min instead of hours typical for enzyme-linked immunosorbent assay applications. Calibration curves of the dependence of a fluorescence signal on the hIgG concentration in a sample have been obtained in one step due to a parallel arrangement of microchannels. A mathematical model of the PA-FITC-hIgG complex formation in the chip has been developed. The values of the kinetic constant of the PA-FITC-hIgG binding (kon=5.5 m3
Czech name
Mikrofluidní čip pro bioanalýzu - určení vazných parametrů
Czech description
Sedmikanálový polystyrenový mikročip byl vyroben mikroobráběním. V čipu byly provedeny jednokroková a sendvičová bioafinitní reakce pro určení koncentrace protilátek ve vzorku. Doba inkubační fáze trvala 5 minut. Byl vytvořen matematický model inkubačnífáze. Optimalizačním výpočtem byly určeny kinetická konstanta tvorby imunoafinitního systému protein A - IgG (kon=5.5 m3 mol-1 s-1) a rozvnovážná disociační konstanta (Kd=3x10-6 mol m-3).
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CI - Industrial chemistry and chemical engineering
OECD FORD branch
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Result continuities
Project
<a href="/en/project/KAN208240651" target="_blank" >KAN208240651: Study of interactions of biological macromolecules and nanolayers with a view to research of polymer microfluidic biosensors and therapeutic nanoparticles</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2007
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biomicrofluidics
ISSN
1932-1058
e-ISSN
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Volume of the periodical
1
Issue of the periodical within the volume
2
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
024101-024101
UT code for WoS article
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EID of the result in the Scopus database
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