SANDWICH BIOASSAYS IN A POLYSTYRENE MICROCHIP

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F07%3A00018093" target="_blank" >RIV/60461373:22340/07:00018093 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

SANDWICH BIOASSAYS IN A POLYSTYRENE MICROCHIP

Original language description

Microfluidic protein chips are devices based on the complementary reaction between a ligand and a receptor. The characteristic size of the devices is in order of microns. This work is focused on study of bioaffinity assays in a polystyrene microchip. Twodifferent arrangements for quantification of human immunoglobulins (hIgG) are described. In both cases, the assays are non-competitive and heterogeneous with protein A (PA) immobilized on polystyrene by a passive sorption as receptors. PA binds the Fc region of human immunoglobulins (ligand). In the one step assay, immobilized PA binds fluorescently labeled hIgG; in the sandwich assay, hIgG is bound as a primary ligand and then a fluorescently labeled anti-human goat IgG as the detection antibody. A mathematical model of the incubation phase of the one-step assay is focused on determination of the kinetic constants of heterogeneous PA-hIgG binding in the polystyrene microfluidic chip.

Czech name

Sendvičová bioanalýza v polystyrénovém mikročipu

Czech description

Byly studovány dvě experimentální uspořádání pro kvantifikaci lidských protilátek ze vzorku. Uspořádání byly nekompetitivní a heterogenní. Receptorem byl protein A a ligandem lidská protilátka. Detekce vzniklého komplexu byla prováděna fluorescenčně. Pomocí matematického modelu byly odhadnuty hodnoty kinetické a rovnovážné konstanty systémů Protein A - IgG.

Type

D - Article in proceedings

CEP classification

CI - Industrial chemistry and chemical engineering

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2007

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Proceedings of 34th International Conference of Slovak Society of Chemical Engineering, 21.-25.5.2007, Tatranské Matliare, Slovenská republika

ISBN

978-80-227-2640-5

ISSN

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e-ISSN

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Number of pages

6

Pages from-to

1-6

Publisher name

Slovenská společnost chemického inženýrství

Place of publication

Bratislava

Event location

Tatranské Matliare, Slovakia

Event date

May 21, 2007

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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