Development of a microfluidic chip for heterogeneous immunoassay

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F06%3A00016651" target="_blank" >RIV/60461373:22340/06:00016651 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Development of a microfluidic chip for heterogeneous immunoassay

Original language description

This work is focused on development of an experimental microchip for heterogeneous immunoassay. Polystyrene substrate is used as the material for fabrication of the microfluidic chip. Techniques of hot embossing and micro-milling are employed in the fabrication. The central part of the chip contains immobilized protein molecules with biological affinity (Protein A - PA). Two procedures of PA immobilization have been studied: (i) passive sorption and (ii) covalent crosslinking of protein layers. It has been found that both these methods are proper. In the one-step assay, liquid samples containing the complementary molecules (human immunoglobulins FITC labeled - hIgG) are dosed in the place of the immobilized layer through the inlet/outlet microfluidic channels. A sandwich (multi-step) system Protein A - human immunoglobulins - goat immunoglobulins (FITC labeled - gIgG) is also studied. After washing out, the fluorescence intensity in microchannels is proportional to the amount of the fo

Czech name

Vývoj mikrofluidního čipu pro heterogenní imunoanalýzu

Czech description

Tato práce je zaměřena na vývoj experimentálního čipu pro heterogenní imunoanalýzu. Polystyren byl použit jako substrát pro výrobu čipu. Při výrobě byly použity techniky mikrofrézování a slinování za tepla. Byly studovány dvě metody detekce biologickýchmolekul - lidských IgG: (i) jednokroková ProteinA-lidský IgG-FITC a (ii) sendvičová ProteinA - lidský IgG - kozí IgG-FITC. Byla vyvinuta imobiizační procedura a oběma technikami byly estrojeny kalibrační křivky závislosti fluorescenčního signálu na koncentraci IgG ve vzorku.

Type

D - Article in proceedings

CEP classification

CI - Industrial chemistry and chemical engineering

OECD FORD branch

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Project

<a href="/en/project/GP104%2F03%2FD006" target="_blank" >GP104/03/D006: Study of microreactors for bioengineering application</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2006

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Sborník 17th International Congress of Chemical and Process Engineering

ISBN

80-86059-45-6

ISSN

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e-ISSN

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Number of pages

7

Pages from-to

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Publisher name

Czech Society of Chemical Engineering

Place of publication

Praha

Event location

Praha

Event date

Aug 27, 2006

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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