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Vibrational circular dichroism study of polypeptide model-membrane systems

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F12%3A43893351" target="_blank" >RIV/60461373:22340/12:43893351 - isvavai.cz</a>

  • Result on the web

    <a href="http://dx.doi.org/10.1016/j.ab.2012.03.023" target="_blank" >http://dx.doi.org/10.1016/j.ab.2012.03.023</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.ab.2012.03.023" target="_blank" >10.1016/j.ab.2012.03.023</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Vibrational circular dichroism study of polypeptide model-membrane systems

  • Original language description

    In this article, we describe the mutual structural effect of the interaction between the model membranes and polylysine and poly-L-arginine. Vibrational circular dichroism (VCD), a method exceptionally sensitive to the polypeptide structure that has notbeen established in such studies before, was the primary method of this study. A complementary technique, electronic circular dichroism, was applied to verify the newly obtained results and as a bridge to the previous studies. We used micelles composed of sodium dodecyl sulfate (SDS) as a monolayer membrane model and large unilamellar vesicles composed of phospholipids as a bilayer membrane model. We describe the conformational changes of the polypeptides caused by the interaction with the model membranes. Among others, the presence of the liposomes in the solution generated special conditions for the formation of the alpha-helical structure of poly-L-arginine; the presence of SDS induced the formation of the beta-structure of polylysin

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CB - Analytical chemistry, separation

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    S - Specificky vyzkum na vysokych skolach

Others

  • Publication year

    2012

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Analytical Biochemistry

  • ISSN

    0003-2697

  • e-ISSN

  • Volume of the periodical

    427

  • Issue of the periodical within the volume

    2

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    8

  • Pages from-to

    211-218

  • UT code for WoS article

    000306872200019

  • EID of the result in the Scopus database