Pilot-scale verification of maximum tolerable hydrodynamic stress for mammalian cell culture

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F16%3A43902731" target="_blank" >RIV/60461373:22340/16:43902731 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1007/s00253-015-7193-x" target="_blank" >http://dx.doi.org/10.1007/s00253-015-7193-x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00253-015-7193-x" target="_blank" >10.1007/s00253-015-7193-x</a>

Result language

angličtina

Original language name

Pilot-scale verification of maximum tolerable hydrodynamic stress for mammalian cell culture

Original language description

Although several scaling bioreactor models of mammalian cell cultures are suggested and described in the literature, they mostly lack a significant validation at pilot or manufacturing scale. The aim of this study is to validate an oscillating hydrodynamic stress loop system developed earlier by our group for the evaluation of the maximum operating range for stirring, based on a maximum tolerable hydrodynamic stress. A 300-L pilot-scale bioreactor for cultivation of a Sp2/0 cell line was used for this purpose. Prior to cultivations, a stress-sensitive particulate system was applied to determine the stress values generated by stirring and sparging. Pilot-scale data, collected from 7- to 28-Pa maximum stress conditions, were compared with data from classical 3-L cultivations and cultivations from the oscillating stress loop system. Results for the growth behavior, analyzed metabolites, productivity, and product quality showed a dependency on the different environmental stress conditions but not on reactor size. Pilot-scale conditions were very similar to those generated in the oscillating stress loop model confirming its predictive capability, including conditions at the edge of failure

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CI - Industrial chemistry and chemical engineering

OECD FORD branch

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Project

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Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Applied Microbiology and Biotechnology

ISSN

0175-7598

e-ISSN

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Volume of the periodical

100

Issue of the periodical within the volume

8

Country of publishing house

US - UNITED STATES

Number of pages

10

Pages from-to

3489-3498

UT code for WoS article

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EID of the result in the Scopus database

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