Characterization of hydrodynamic stress in ambr250 (R) bioreactor system and its impact on mammalian cell culture

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F22%3A43925005" target="_blank" >RIV/60461373:22340/22:43925005 - isvavai.cz</a>

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S1369703X21003168?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1369703X21003168?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bej.2021.108240" target="_blank" >10.1016/j.bej.2021.108240</a>

Result language

angličtina

Original language name

Characterization of hydrodynamic stress in ambr250 (R) bioreactor system and its impact on mammalian cell culture

Original language description

During the cell culture in stirred aerated vessels, both stirring and sparging is required for proper homogenization of a mixture and oxygen delivery. Quality by design (QbD) in biopharmaceutical industry aims at identification of the impact of mass transfer, dissolved oxygen, pH and hydrodynamic stress on the cell behavior, productivity and product quality. Most of these properties are influenced by impeller motion and sparger aeration. In this research, the shear sensitive poly(methyl methacrylate) (PMMA) aggregates are used as probes capable of determining the maximum hydrodynamic stress (tau(max)) present in the ambr250 (R) bioreactor. The results from our micro-aggregate probes are connected with the cultivation data using two Chinese hamster ovary K1 glutamine synthetase mediated cell lines (CHO-K1 GS) to determine the stress threshold, below which no significant impact of operating conditions on cell behavior is observed. Such calibrated system can be in the future used as a scale-down model of large-scale bioreactors to test cell robustness during process development.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

20401 - Chemical engineering (plants, products)

Project

—

Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biochemical Engineering Journal

ISSN

1369-703X

e-ISSN

1873-295X

Volume of the periodical

177

Issue of the periodical within the volume

JAN 2022

Country of publishing house

BE - BELGIUM

Number of pages

9

Pages from-to

108240

UT code for WoS article

000717092700010

EID of the result in the Scopus database

2-s2.0-85118537595