Investigation of poloxamer cell protective ability via shear sensitive aggregates in stirred aerated bioreactor
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F22%3A43925033" target="_blank" >RIV/60461373:22340/22:43925033 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S1369703X22002182?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1369703X22002182?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.bej.2022.108549" target="_blank" >10.1016/j.bej.2022.108549</a>
Alternative languages
Result language
angličtina
Original language name
Investigation of poloxamer cell protective ability via shear sensitive aggregates in stirred aerated bioreactor
Original language description
Mammalian cell cultivation in bioreactors is widely practiced in the pharmaceutical industry for production of monoclonal antibodies, vaccines and other therapeutic products. During cell culture, ensuring homogeneity and oxygen transport to the cultivation broth is essential for survival and proper cell growth. Due to the lack of a cell wall, mammalian cells are prone to damage by the gas aeration. Common practice how to protect mammalian cells against hydrodynamic forces is usage of poloxamers – synthetic, water-soluble nonionic copolymers. However, poloxamers are never pure compounds and the distribution of molecular weight strongly depends on the production process. This may lead to the final product containing impurities, which may have a negative impact on the protectivity of cells. Therefore, it is of high importance to know whether a particular poloxamer can be used as a cell protective agent. In this paper, we extend the usability of our method based on shear sensitive poly(methyl methacrylate) (PMMA) aggregate probes to investigate the protective capability of poloxamers. Comparative study of three different poloxamers and their impact on maximum effective hydrodynamic stress (τmax) and mass transfer coefficient (kLa) is conducted in the bench-scale 3.5 L Minifors bioreactor.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
20401 - Chemical engineering (plants, products)
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biochemical Engineering Journal
ISSN
1369-703X
e-ISSN
1873-295X
Volume of the periodical
186
Issue of the periodical within the volume
AUG 2022
Country of publishing house
BE - BELGIUM
Number of pages
7
Pages from-to
108549
UT code for WoS article
000891992900007
EID of the result in the Scopus database
2-s2.0-85135357154