Chiroptical spectroscopy of human blood plasma: Revealing the signature of degenerative diseases

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F16%3A43903344" target="_blank" >RIV/60461373:22340/16:43903344 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Chiroptical spectroscopy of human blood plasma: Revealing the signature of degenerative diseases

Original language description

The greatest advantage of chiroptical spectroscopy is its inherent sensitivity to the structure and conformation of chiral biomolecules, such as peptides, proteins, saccharides or nucleic acids. However except our recent studies, it has never been used for a systematic analysis of biofluids. As blood-based testing represents a basis for a future sensitive screening of populations at risk of various degenerative diseases, we focus on the analysis of blood plasma using Raman optical activity (ROA) and electronic circular dichroism. To gain more detailed structure information, chiroptical methods are supplemented by conventional Raman and infrared spectroscopies. Unfortunately, blood plasma is rich in molecules that may generate a background of large amplitude overlapping the desired ROA and Raman signals. To enable the measurements and to improve the reliability of the data acquired, we developed a methodology for the minimization of background fluorescence. Using the combination of the above mentioned methods, we monitored the specific molecular signature of blood plasma components and the differences thereof in healthy subjects and patients with pancreatic cancer and diabetes mellitus. Focusing on the spectral regions that reflect the structure of essential biomolecules, the obtained data sets were statistically evaluated yielding high values of specificity and sensitivity. The achieved results indicate a promising potential of the combination of chiroptical and vibrational spectroscopies as a powerful tool in the identification of disease-related structural changes of chiral biomolecules within blood plasma.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

<a href="/en/project/NV16-31028A" target="_blank" >NV16-31028A: Efficacy of new spectroscopic biomarkers for detection of early stage pancreatic cancer</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů