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Spheroid cultivation of HT-29 carcinoma cell line in liquid marbles

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F17%3A43913498" target="_blank" >RIV/60461373:22340/17:43913498 - isvavai.cz</a>

  • Alternative codes found

    RIV/68378050:_____/17:00507306

  • Result on the web

    <a href="http://dx.doi.org/10.1007/s11696-016-0026-2" target="_blank" >http://dx.doi.org/10.1007/s11696-016-0026-2</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1007/s11696-016-0026-2" target="_blank" >10.1007/s11696-016-0026-2</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Spheroid cultivation of HT-29 carcinoma cell line in liquid marbles

  • Original language description

    The ability to simulate the 3D structure of a human body is essential to increase the efficiency of drug development. In vivo conditions are significantly different in comparison to in vitro conditions. A standardly used cell monolayer on tissue culture plastic (2D cell culture) is not sufficient to simulate the transfer phenomena occurring in living organisms, therefore, cell growth in a 3D space is desired. Drug absorption, distribution, metabolism, excretion and toxicity could be tested on 3D cell aggregates called spheroids, decrease the use of animal models and accelerate the drug development. In this work, the formation of spheroids from HT-29 human colorectal adenocarcinoma cells was successfully achieved by means of the so-called liquid marbles, which are liquid droplets encapsulated by a hydrophobic powder. During the cultivation in the medium inside the liquid marbles, cells spontaneously formed spherical agglomerates (spheroids) without the need of any supporting scaffold. The study focused on the influence of different parameters-namely liquid marble volume, seeding cell density and time of cultivation-on the final yield and quality of spheroids. This work has shown that using liquid marbles as microbioreactors is a suitable method for the cultivation of HT-29 cells in the form of spheroids.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    20402 - Chemical process engineering

Result continuities

  • Project

    <a href="/en/project/NV16-34342A" target="_blank" >NV16-34342A: Multifunctional Nano-Clusters as a Drug Delivery System</a><br>

  • Continuities

    S - Specificky vyzkum na vysokych skolach

Others

  • Publication year

    2017

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Chemical Papers

  • ISSN

    0366-6352

  • e-ISSN

  • Volume of the periodical

    71

  • Issue of the periodical within the volume

    6

  • Country of publishing house

    SK - SLOVAKIA

  • Number of pages

    9

  • Pages from-to

    1055-1063

  • UT code for WoS article

    000401069700006

  • EID of the result in the Scopus database