Spheroid cultivation of HT-29 carcinoma cell line in liquid marbles
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F17%3A00507306" target="_blank" >RIV/68378050:_____/17:00507306 - isvavai.cz</a>
Alternative codes found
RIV/60461373:22340/17:43913498
Result on the web
<a href="https://link.springer.com/article/10.1007%2Fs11696-016-0026-2" target="_blank" >https://link.springer.com/article/10.1007%2Fs11696-016-0026-2</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s11696-016-0026-2" target="_blank" >10.1007/s11696-016-0026-2</a>
Alternative languages
Result language
angličtina
Original language name
Spheroid cultivation of HT-29 carcinoma cell line in liquid marbles
Original language description
The ability to simulate the 3D structure of a human body is essential to increase the efficiency of drug development. In vivo conditions are significantly different in comparison to in vitro conditions. A standardly used cell monolayer on tissue culture plastic (2D cell culture) is not sufficient to simulate the transfer phenomena occurring in living organisms, therefore, cell growth in a 3D space is desired. Drug absorption, distribution, metabolism, excretion and toxicity could be tested on 3D cell aggregates called spheroids, decrease the use of animal models and accelerate the drug development. In this work, the formation of spheroids from HT-29 human colorectal adenocarcinoma cells was successfully achieved by means of the so-called liquid marbles, which are liquid droplets encapsulated by a hydrophobic powder. During the cultivation in the medium inside the liquid marbles, cells spontaneously formed spherical agglomerates (spheroids) without the need of any supporting scaffold. The study focused on the influence of different parameters-namely liquid marble volume, seeding cell density and time of cultivation-on the final yield and quality of spheroids. This work has shown that using liquid marbles as microbioreactors is a suitable method for the cultivation of HT-29 cells in the form of spheroids.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Chemical Papers
ISSN
0366-6352
e-ISSN
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Volume of the periodical
71
Issue of the periodical within the volume
6
Country of publishing house
CH - SWITZERLAND
Number of pages
9
Pages from-to
1055-1063
UT code for WoS article
000401069700006
EID of the result in the Scopus database
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