Dynamic behaviour of single and coupled glycolytic oscillators

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F18%3A43917067" target="_blank" >RIV/60461373:22340/18:43917067 - isvavai.cz</a>
Result on the web
<a href="http://www.socphyschemserb.org/en/events/pc2018/" target="_blank" >http://www.socphyschemserb.org/en/events/pc2018/</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Dynamic behaviour of single and coupled glycolytic oscillators
Original language description
Our study is focused on experimental observations of dynamic behaviour of glycolytic oscillatory reaction using yeast extract and glucose solution reaction in a single cell and two coupled cells via reciprocal peristaltic pumping. Dynamic oscillatory behaviour of each cell is measured using UV-vis spectrophotometer, specifically 340nm for NADH and 260nm for adenosine metabolic pool and NAD+. We report occurrence of oscillation of NADH, excitation of NADH and bistability connected with depletion of adenosine metabolic pool. The other part of our study is focused on modelling of ring of twenty coupled cells, using core model of glycolysis proposed by Moran and Goldbeter. Transport rate coefficients are set to be equal. The coexistence of nonuniform stationary states and oscillatory region is shown in solution diagram in a plane of concentration of ADP in the first reactor and inhibition rate coefficient.
Czech name
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Czech description
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Project
<a href="/en/project/GA18-24397S" target="_blank" >GA18-24397S: Constrained analysis of reaction networks - a tool for experimental validation of models of biochemical and photobiological reactors</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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