Comparative Study on Handheld, Modular, and Laboratory Raman Instruments for the Analysis of Colon Tissues and Colorectal Polyps

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F24%3A43927927" target="_blank" >RIV/60461373:22340/24:43927927 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11110/24:10478247 RIV/00064165:_____/24:10478247

Result on the web

<a href="https://doi.org/10.3390/app14020791" target="_blank" >https://doi.org/10.3390/app14020791</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/app14020791" target="_blank" >10.3390/app14020791</a>

Result language

angličtina

Original language name

Comparative Study on Handheld, Modular, and Laboratory Raman Instruments for the Analysis of Colon Tissues and Colorectal Polyps

Original language description

Portable Raman spectrometers may offer advantages for clinical medical diagnostics overlaboratory instruments by allowing for quick measurements in the field and provision of data suitablefor screening analyses. This work evaluates the potential of using available handheld, modular,and laboratory Raman spectrometers for screening normal colon tissues and benign and malignantcolon polyps. The Raman spectra of tissue samples and reference biological macromolecules weremeasured with these instruments and analyzed using curve fitting and multivariate statistics. Thespectra of calf thymus DNA measured with portable devices showed suitable signal-to-noise levelsand half-widths of the prominent bands. Band positions, resolution, and relative intensities inthe Raman spectra of colon tissues and reference compounds varied for the instruments, and thelaboratory device demonstrated the best spectral feature. The principal component analysis (PCA)of the spectra obtained with all Raman devices showed well discrimination of normal colon tissue,adenomatous polyp, and adenocarcinoma. Dendrograms of similarity obtained using hierarchycluster analysis (HCA) for the Raman spectra of all three devices also showed good separation ofthese samples. The soft independent modeling of class analogy (SIMCA) and support vector machine(SVM) models efficiently classified normal colon tissues and benign/malignant colorectal polypsbased on the Raman data from all three devices. Despite its less pronounced spectral characteristics,the handheld Raman spectrometer can be used in early diagnosis of colorectal carcinoma, comparableto the modular and laboratory instruments.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30219 - Gastroenterology and hepatology

Project

<a href="/en/project/NU20-09-00229" target="_blank" >NU20-09-00229: The development of novel analytical approaches for early diagnosis of adenomatous polyps and prevention of colorectal carcinoma</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Applied Sciences-Basel

ISSN

2076-3417

e-ISSN

2076-3417

Volume of the periodical

14

Issue of the periodical within the volume

2

Country of publishing house

CH - SWITZERLAND

Number of pages

19

Pages from-to

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UT code for WoS article

001149241300001

EID of the result in the Scopus database

2-s2.0-85192433337