Circulating Endometrial Cells: A New Source of Information on Endometriosis Dynamics

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61383082%3A_____%2F19%3A00000500" target="_blank" >RIV/61383082:_____/19:00000500 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11110/19:10400781 RIV/00216208:11120/19:43919335 RIV/00216208:11140/19:10400781 RIV/00064173:_____/19:N0000029 and 2 more

Result on the web

<a href="https://www.mdpi.com/journal/jcm" target="_blank" >https://www.mdpi.com/journal/jcm</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/jcm8111938" target="_blank" >10.3390/jcm8111938</a>

Result language

angličtina

Original language name

Circulating Endometrial Cells: A New Source of Information on Endometriosis Dynamics

Original language description

The focus of the presented work was to isolate and characterize circulating endometrial cells (CECs) enriched from peripheral blood (PB) of patients with diagnosed endometriosis. The molecular characteristics of CECs could be supportive for an understanding of endometriosis pathogenesis and treatment decisions in the future. Material and Methods: Blood samples (n = 423) were tested for CECs presence. Subsequently, gene expression analysis (GEA) was carried out for CECs. In parallel, CECs presence and characteristics were tested during menstrual cycle (MC) phases in 11 patients. CECs were enriched by size-based separation. Results: CECs were present in 78.4% of the tested blood samples. In line with the revised American Fertility Society (rAFS) classification, CECs presence was confirmed in all the acknowledged endometriosis stages: minimal, mild, moderate, and severe. Surprisingly, CECs negativity rate was also reported for severe disease in 21.1% of cases. The CECs captured during MC phases displayed different cytomorphology, including epithelial, stromal, and stem cell-like characteristics. The highest CECs numbers were detected in the mid-secretory phase of MC, which corresponds to uterine lining decidualization. CECs captured during mid-secretory periods expressed genes KRT18, NANOG, and VIM in higher amounts when compared to the proliferative phase of MC, where genes KRT19 and ESR1 were mostly elevated. GEA of the super-positive CECs samples (1000 CECs/8 mL PB) revealed high expression of genes KRT18, VIM, NANOG, and FLT1. The expression of these genes was also elevated in the endometriosis tissue samples and endometrioma. Conclusion: The panel of the identified CEC genes could be tested in a prospective manner to confirm the role of CECs in endometriosis pathogenesis and diagnostics.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30200 - Clinical medicine

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

JOURNAL OF CLINICAL MEDICINE

ISSN

2077-0383

e-ISSN

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Volume of the periodical

8

Issue of the periodical within the volume

11

Country of publishing house

CH - SWITZERLAND

Number of pages

1

Pages from-to

"nestránkováno"

UT code for WoS article

000502294400181

EID of the result in the Scopus database

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