Morphology of mitochondrial network in disseminated endometriosis cells in spontaneous pneumothorax diagnostic process

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61383082%3A_____%2F24%3A00001440" target="_blank" >RIV/61383082:_____/24:00001440 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11110/24:10486712

Result on the web

<a href="https://pubmed.ncbi.nlm.nih.gov/39605127/" target="_blank" >https://pubmed.ncbi.nlm.nih.gov/39605127/</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.14715/cmb/2024.70.10.4" target="_blank" >10.14715/cmb/2024.70.10.4</a>

Result language

angličtina

Original language name

Morphology of mitochondrial network in disseminated endometriosis cells in spontaneous pneumothorax diagnostic process

Original language description

Circulating endometrial cells (CECs) have emerged as a new biomarker of advanced disease in women with endometriosis. The identification of several subtypes of CECs (e.g., stem cell-like, epithelial, glandular, stromal) has opened the way for characterization of endometriosis-associated CECs. This study focused on the isolation and characterization of CECs and disseminated endometrial cells (DECs) in patients with spontaneous pneumothorax (SP). The primary objective was to differentiate between cancer and non-cancer cells in patients with no previous cancer diagnosis. The MetaCell® size-based separation protocol was used to enrich CECs/DECs. Evaluation of the captured cells by 3D microscopy was performed using a NANOLIVE™ microscope using a holographic approach. Based on gene expression analysis (GEA), we can conclude that mitochondria are much more active in primary tumors compared to endometriosis tissue (e.g. MT-ND1, MTATP6 genes). The culture of DECs is made of stromal, stem and immune cells. In vitro culture of DECs is characterized by an increase in the epithelial marker KRT18. Similarly, NFE2L2, a proerythroid factor, is also elevated. Further, a significant decrease in the amount of stem and immune cells was observed in the cell culture of DECs. The data presented here show how morphologically plastic the changes in the mitochondrial network can be and how cells can reflect them at the level of gene expression. The markers identified could help in the accompanying diagnostic process of the spontaneous pneumothorax in women of reproductive age.

Czech name

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Czech description

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Type

J_SC - Article in a specialist periodical, which is included in the SCOPUS database

CEP classification

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OECD FORD branch

30214 - Obstetrics and gynaecology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Cellular and Molecular Biology

ISSN

0145-5680

e-ISSN

—

Volume of the periodical

70

Issue of the periodical within the volume

10

Country of publishing house

FR - FRANCE

Number of pages

5

Pages from-to

25-29

UT code for WoS article

—

EID of the result in the Scopus database

2-s2.0-85211067351