Monitoring of monkeypox viral DNA in Prague wastewater

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61383082%3A_____%2F23%3A00001290" target="_blank" >RIV/61383082:_____/23:00001290 - isvavai.cz</a>

Alternative codes found

RIV/60162694:G33__/23:N0000015 RIV/60162694:G44__/24:00560309 RIV/00216208:11110/23:10467756 RIV/60461373:22320/23:43927063 RIV/60461373:22330/23:43927063

Result on the web

<a href="https://pubmed.ncbi.nlm.nih.gov/37567313/" target="_blank" >https://pubmed.ncbi.nlm.nih.gov/37567313/</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.scitotenv.2023.166110" target="_blank" >10.1016/j.scitotenv.2023.166110</a>

Result language

angličtina

Original language name

Monitoring of monkeypox viral DNA in Prague wastewater

Original language description

Monkeypox virus (Mpxv) is a dsDNA virus that has become a global concern for human health in 2022. As both infected people and non-human hosts can shed the virus from their skin, faeces, urine and other body fluids, and the resulting sewage contains viral load representative of the whole population, it is highly promising to detect the spread of monkeypox virus in municipal wastewater. We established a methodology for sewage-based monitoring of Mpxv in Prague and analysed samples (n = 24) already early August–October of 2022 in a municipality with 1.4 million inhabitants that only reported 29 cumulative cases in this period. We isolated Mpxv DNA with the Wizard Enviro Total Nucleic Acid Kit, and thereafter detected Mpxv DNA using the EliGene® Monkeypox RT-PCR Kit. Prague wastewater was positive for Mpxv (in total 9 positive samples in periods with 1—9 new cases per week, coinciding with a weekly incidence of 0.07–0.64 per 100,000 inhabitants. The method for confirmation of wastewater positivity via semi-nested PCR and Sanger sequencing was successfully confirmed on positive controls including Mpxv particles and Mpxv-positive wastewater from the Netherlands. However, for Prague wastewater samples, amplification of Mpxv DNA via semi-semi-nested PCR was unsuccessful. This was probably due to extremely low case count, leading to the amplification of non-target bacterial DNA. Compared to other studies with much higher Mpxv prevalence, we show the outstanding sensitivity of our approach for monitoring the spread of monkeypox using wastewater.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30304 - Public and environmental health

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

SCIENCE OF THE TOTAL ENVIRONMENT

ISSN

0048-9697

e-ISSN

—

Volume of the periodical

902

Issue of the periodical within the volume

Aug

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

6

Pages from-to

1-6

UT code for WoS article

001057942100001

EID of the result in the Scopus database

2-s2.0-85167795638