Comparison of three template preparation methods for routine detection of beak and feather disease virus and avian polyomavirus with single and nested polymerase chain reaction in clinical specimens.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70565813%3A_____%2F08%3A%230000117" target="_blank" >RIV/70565813:_____/08:#0000117 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Comparison of three template preparation methods for routine detection of beak and feather disease virus and avian polyomavirus with single and nested polymerase chain reaction in clinical specimens.

Original language description

Three DNA extraction methods were compared for routine polymerase chain reaction (PCR) detection of beak and feather disease virus (BFDV) in whole blood and feather samples and of avian polyomavirus (APV) in feather samples. Boiling in Chelex 100 Resin was found to be the most sensitive method for detection of BFDV or APV DNA in both feather and blood samples. The lower rate of BFDV DNA detection when using NucleoSpin kits was not a result of inhibition of PCR in most cases. Inhibition of DNA amplification was observed when using the DNAzol Direct kit. Therefore, of the methods evaluated here, Chelex 100 Resin treatment of samples was the best option for routine testing for BFDV and APV DNA in clinical samples.

Czech name

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Czech description

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Type

J_ost - Miscellaneous article in a specialist periodical

CEP classification

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OECD FORD branch

40301 - Veterinary science

Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2008

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Avian pathology

ISSN

0307-9457

e-ISSN

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Volume of the periodical

37

Issue of the periodical within the volume

2

Country of publishing house

GB - UNITED KINGDOM

Number of pages

5

Pages from-to

145-149

UT code for WoS article

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EID of the result in the Scopus database

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