Development and evaluation of TaqMan real-time PCR assay for detection of beak and feather disease virus

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F17%3A43875823" target="_blank" >RIV/62157124:16270/17:43875823 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.jviromet.2017.02.017" target="_blank" >http://dx.doi.org/10.1016/j.jviromet.2017.02.017</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jviromet.2017.02.017" target="_blank" >10.1016/j.jviromet.2017.02.017</a>

Result language

angličtina

Original language name

Development and evaluation of TaqMan real-time PCR assay for detection of beak and feather disease virus

Original language description

Psittacine beak and feather disease (PBFD) is one of the most significant viral diseases in psittacine birds. The aim of the presented study was to develop a highly specific and sensitive TagMan real-time PCR assay for universal detection of beak and feather disease virus (BFDV). Primers and a hydrolysis probe were selected on the highly conserved regions belonging to the ORF1 of the BFDV genome which were identified by aligning 814 genomic sequences downloaded from the GenBank database. The evaluation of the reaction parameters suggested a reaction efficiency of 97.1%, with consistent detection of 101 virus copies/mu l of nucleic acid extract. The low values of standard deviation and coefficient of variation indicate a high degree of reproducibility and repeatability. The diagnostic applicability of the assay was proven on 36 BFDV positive and 107 negative specimens of psittacine origin representing 28 species. The assay showed a 100% ability to detect distinct genetic variants of the virus. Our data suggest that the presented TaqMan real-time PCR represents a specific, sensitive and reliable assay facilitating the molecular detection of BFDV.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

40301 - Veterinary science

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Virological Methods

ISSN

0166-0934

e-ISSN

—

Volume of the periodical

244

Issue of the periodical within the volume

JUN 2017

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

6

Pages from-to

55-60

UT code for WoS article

000401677200010

EID of the result in the Scopus database

—