Oxidative Stress Markers Are Elevated in Exhaled Breath Condensate of Workers Exposed to Nanoparticles during Iron Oxide Pigment Production.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F16%3A00456197" target="_blank" >RIV/61388955:_____/16:00456197 - isvavai.cz</a>

Alternative codes found

RIV/67985858:_____/16:00456197 RIV/00216208:11110/16:10325510 RIV/00216208:11510/16:10325510 RIV/60461373:22310/16:43902605 RIV/00064165:_____/16:10325510

Result on the web

<a href="http://dx.doi.org/10.1088/1752-7155/10/1/016004" target="_blank" >http://dx.doi.org/10.1088/1752-7155/10/1/016004</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1088/1752-7155/10/1/016004" target="_blank" >10.1088/1752-7155/10/1/016004</a>

Result language

angličtina

Original language name

Oxidative Stress Markers Are Elevated in Exhaled Breath Condensate of Workers Exposed to Nanoparticles during Iron Oxide Pigment Production.

Original language description

Markers of oxidative stress and inflammation were analysed in the exhaled breath condensate (EBC) and urine samples of 14 workers (mean age 43±7 years) exposed to iron oxide aerosol for an average of 10±4 years and 14 controls (mean age 39±4 years) by liquid chromatography-electrospray ionization-mass spectrometry/mass spectrometry (LC-ESI-MS/MS) after solid-phase extraction. Aerosol exposure in the workplace was measured by particle size spectrometers, a scanning mobility particle sizer (SMPS) and an aerodynamic particle sizer (APS), and by aerosol concentration monitors, P-TRAK and DustTRAK DRX. Total aerosol concentrations in workplace locations varied greatly in both time and space. The median mass concentration was 0.083mg m(-3) (IQR 0.063-0.133mg m(-3)) and the median particle concentration was 66800 particles cm(-3) (IQR 16900-86900 particles cm(-3)). In addition, more than 80% of particles were smaller than 100nm in diameter. Markers of oxidative stress, malondialdehyde (MDA), 4-hydroxy-trans-hexenale (HHE), 4-hydroxy-trans-nonenale (HNE), 8-isoProstaglandin F2alpha (8-isoprostane) and aldehydes C6-C12, in addition to markers of nucleic acid oxidation, including 8-hydroxy-2-deoxyguanosine (8-OHdG), 8-hydroxyguanosine (8-OHG), 5-hydroxymethyl uracil (5-OHMeU), and of proteins, such as o-tyrosine (o-Tyr), 3-chlorotyrosine (3-ClTyr), and 3-nitrotyrosine (3-NOTyr) were analysed in EBC and urine by LC-ESI-MS/MS. Almost all markers of lipid, nucleic acid and protein oxidation were elevated in the EBC of workers comparing with control subjects. Elevated markers were MDA, HNE, HHE, C6-C10, 8-isoprostane, 8-OHdG, 8-OHG, 5-OHMeU, 3-ClTyr, 3-NOTyr, o-Tyr (all p<0.001), and C11 (p<0.05). Only aldehyde C12 and the pH of samples did not differ between groups. Markers in urine were not elevated. These findings suggest the adverse effects of nano iron oxide aerosol exposure and support the utility of oxidative stress biomarkers in EBC. n

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10403 - Physical chemistry

Project

<a href="/en/project/LO1215" target="_blank" >LO1215: Prague University Analytical Center for Health, Food Safety and Environmental Protection</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Breath Research

ISSN

1752-7155

e-ISSN

—

Volume of the periodical

10

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

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UT code for WoS article

000375749700006

EID of the result in the Scopus database

2-s2.0-84983215770