Genome-wide C-SWAT library for high-throughput yeast genome tagging
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F18%3A00506546" target="_blank" >RIV/61388955:_____/18:00506546 - isvavai.cz</a>
Result on the web
<a href="http://hdl.handle.net/11104/0297778" target="_blank" >http://hdl.handle.net/11104/0297778</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41592-018-0045-8" target="_blank" >10.1038/s41592-018-0045-8</a>
Alternative languages
Result language
angličtina
Original language name
Genome-wide C-SWAT library for high-throughput yeast genome tagging
Original language description
Here we describe a C-SWAT library for high-throughput tagging of Saccharomyces cerevisiae open reading frames (ORFs). In 5,661 strains, we inserted an acceptor module after each ORF that can be efficiently replaced with tags or regulatory elements. We validated the library with targeted sequencing and tagged the proteome with bright fluorescent proteins to quantify the effect of heterologous transcription terminators on protein expression and to localize previously undetected proteins.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10403 - Physical chemistry
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Nature Methods
ISSN
1548-7091
e-ISSN
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Volume of the periodical
15
Issue of the periodical within the volume
8
Country of publishing house
GB - UNITED KINGDOM
Number of pages
3
Pages from-to
598-600
UT code for WoS article
000440334000019
EID of the result in the Scopus database
2-s2.0-85049640566